Adaptations of neutrophils in cancer.

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Abstract

[Indexed for MEDLINE] Conflict of interest statement: Declaration of interests The authors declare no competing interests. 4. Cytometry B Clin Cytom. 2017 May;92(3):218-227. doi: 10.1002/cyto.b.21219. Epub 2015 Aug 18. Introduction to the diagnosis and classification of monocytic-lineage leukemias by flow cytometry. Matarraz S(1), Almeida J(1), Flores-Montero J(1), Lécrevisse Q(1), Guerri V(1), López A(1), Bárrena S(1), Van Der Velden VHJ(2), Te Marvelde JG(2), Van Dongen JJM(2), Orfao A(1). Author information: (1)Servicio De Citometría (NUCLEUS) and Department of Medicine, Centro De Investigación Del Cáncer (IBMCC, University of Salamanca-CSIC) and IBSAL, Salamanca, Spain. (2)Department of Immunology, Erasmus MC, University Medical Center Rotterdam, Rotterdam, The Netherlands. Despite diagnostic criteria are currently available for the distinct subtypes of monocytic-lineage neoplasias, a number of partially overlapping features still remain evident, which may hamper their differential diagnosis. An accurate identification and characterization of monocytic cells is of major relevance for the diagnosis and classification of these neoplasias. In this regard, as compared to other conventional techniques, flow cytometry has shown the highest sensitivity for detection of early monocytic commitment of (normal and neoplastic) bone marrow CD34+ hematopoietic precursors as well as of monocytic aberrations and maturation blockades, which are frequently associated with clonal myeloid disorders. In the present paper we provide basic principles and criteria for multiparameter flow cytometry identification and characterization of bone marrow monocytic cells that contribute to an improved diagnostic and classification of monocytic lineage-associated acute leukemias in clinical settings, particularly when using the EuroFlow antibody panels. © 2015 International Clinical Cytometry Society. © 2015 International Clinical Cytometry Society. DOI: 10.1002/cyto.b.21219