Integrative Metabolomics and Pharmacology Reveal Guzhi Zengsheng Zhitongwan's Mechanism in Knee Osteoarthritis.

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Abstract

[Indexed for MEDLINE] Conflict of interest statement: Declaration of competing interest We declare that all authors have no conflicts of interest that could affect the objectivity of the research. We have fully disclosed any potential conflicts of interest and ensure that these conflicts do not influence the design, execution, or reporting of the study. 8. Medicine (Baltimore). 2019 Jul;98(29):e16501. doi: 10.1097/MD.0000000000016501. bbFISH-ing in the sonication fluid. Birlutiu RM(1), Birlutiu V(2), Cismasiu RS(3), Mihalache M(4). Author information: (1)Lucian Blaga University of Sibiu, Faculty of Medicine Sibiu; FOISOR Clinical Hospital of Orthopedics, Traumatology, and Osteoarticular TB Bucharest. (2)Lucian Blaga University of Sibiu, Faculty of Medicine Sibiu; Academic Emergency Hospital Sibiu-Infectious Diseases Clinic, Sibiu. (3)Carol Davila University of Medicine and Pharmacy Bucharest, Romania; FOISOR Clinical Hospital of Orthopedics, Traumatology, and Osteoarticular TB Bucharest. (4)Lucian Blaga University of Sibiu, Faculty of Medicine Sibiu, Romania. By 2030, the annual number of combined total hip and knee arthroplasty is estimated to reach 3.5 to 4 million in the US alone. In the context of a constant increase of the number of primary and revision total hip and knee arthroplasty, an increased risk of complication is expected. Prosthetic joint infections (PJIs) represent major cause of healthcare expenditure and morbidity. PJI still remain the most common and feared arthroplasty complication. A rapid and correct diagnosis of infection is decisive for a correct therapeutical management. In this setting, the Academic Emergency Hospital Sibiu adopted and implemented, with the beginning of September 2016, a new strategy for the diagnosis of PJIs strategy that uses sonication and beacon-based fluorescent in situ hybridization (bbFISH) technology.Until November 2017, 40 patients (40 retrieved implants) were enrolled in the study. Sonication fluid (SF) was collected after sonication of the implants, and samples were harvested on aerobic and anaerobic culture media. A bbFISH was used as a rapid method of bacteria detection.16 patients were diagnosed with PJIs (all 16 patients presented a positive culture of the SF). Comparing bbFISH with culture, 11 samples tested true-positive. As the kit doesn't contain probes for Pseudomonas fluorescens or Ralstonia pickettii, 4 strains of R pickettii and 1 strain of P fluorescens that was associated with Staphylococcus epidermidis were not detected.Bacteria culture of SF remains the gold standard. bbFISH holds promise to be a diagnostic tool for rapid identifying of PJIs. The bbFISH assay needs to be optimized for the detection of bacterial strains that are relevant for the PJIs field. DOI: 10.1097/MD.0000000000016501 PMCID: PMC6709156